Dunaliella pseudosalina D-701

History of Receipt and Isolation

• Country of origin: Mongolia
• Place of isolation: Provided by the collection of the Institute of Biotechnology, Academy of Sciences
• Last determination: Zayadan B.K., Akmukhanova N.R. in the Laboratory of Biotechnology of al-Farabi Kazakh National University, 2015
• Year of accession to the collection: 2001
• Method of obtainment: collection strain
• Genetic features: wild type
• Culture condition: axenic

Morphology and Structure

a unicellular motile organism with green or yellow-green coloration depending on cultivation conditions. The cells are oval or broadly ovoid, approximately 11–23 µm long and 6–16 µm wide. Each cell contains one large cup-shaped chloroplast with a distinct pyrenoid. Two flagella are located at the anterior end of the cell and are approximately 1.5 times the cell length.

Cultivation

• Culture medium: Artari mineral medium (liquid and agarized)
• Temperature: 26–28 °C
• Light intensity: 50–100 µmol photons/m²/s
• Light regime: 16:8 h (light/dark)
• pH: 6.5–7.0

Physiological Characteristics

heterothallic halophile, mesophile, predominantly thermophile, photophilic autotroph; rich in β-carotene.

Long-term Preservation Conditions

Stored on liquid and 1.5–2.0 % agarized Artari mineral medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Field of Application

In fundamental research and biotechnology.