Oscillatoria subbrevis CZS 2201

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: hot springs of Chundzha, Almaty Region
• Habitat: Thermal aquatic ecosystem.
• Isolator(s): Sandybayeva S.K., Zayadan B.K., Kakimova A.B., Bolatkhan K., Toktybay A.K., Seiilbek S.N. at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Year of accession to the collection: 2023.
• Method of strain obtainment: isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking
• Genetic features: Wild type (WT)
• Strain condition: axenic.

Morphology and Structure

Filamentous cyanobacterium without heterocytes. Trichomes are solitary, almost straight, not tapering at the apex and not constricted at the cross-walls. Cells are 1.5–3.1 µm long and 6.5–7.1 µm wide, without granulation at the cross-walls. The terminal cell is rounded; calyptra is absent.

Cultivation

• Nutrient medium: Zarrouk medium; also grows on BG-11 medium
• Temperature: 32 °C
• Light intensity: 62 µmol photons·m⁻²·s⁻¹
• Photoperiod: 12:12 h (light/dark)
• pH: 8.5

Physiological Characteristics

aerobic phototroph

Long-term Preservation Conditions

Maintained in liquid or agarized Zarrouk medium with transfer every 2–3 months. Dried biomass is stored at 4 °C in darkness under dry conditions.

Deposition Information

Certificate of deposit No. 09/12-453 dated October 19, 2023. Registration Number: RKM 1063

Genetic Information

Molecular identification was based on 16S rRNA gene sequencing using primers 27F/1492R. GenBank accession: OQ627016.

Field of Application

Studies of thermotolerant cyanobacteria, pigments and antioxidant metabolites; production of biomass for analytical and biotechnological screening.

Bibliography

1. Sandybayeva, S.K.; Kossalbayev, B.D.; Zayadan, B.K.; Kopecký, J.; Kakimova, A.B.; Bolatkhan, K.; Allakhverdiev, S.I. Isolation, Identification and Pigment Analysis of Novel Cyanobacterial Strains from Thermal Springs. Plants 2024, 13, 2951. https://doi.org/10.3390/plants13212951

Patents and Other Intellectual Property

Patent for utility model “Cyanobacterium Oscillatoria subbrevis CZS 2201 strain used as a raw material for production of glycosylated carotenoid – myxoxanthophyll” No. 9607 dated September 27, 2024.