Oscillatoria brevis SH-12

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Site of isolation: village of Avangard, Zhanakorgan District, Kyzylorda Region
• Habitat: rice fields and aquatic ecosystems
• Isolator(s): Sadvakasova A.K., Zayadan B.K., Kossalbayev B.D., Bauenova M.O. at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Year of accession to the collection: 2022
• Last identification: Sadvakasova A.K., Zayadan B.K., Kossalbayev B.D., Bauenova M.O., 2023
• Method of obtainment: isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking
• Genetic features: Wild type (WT)
• Culture condition: Axenic

Morphology and Structure

Trichomes are filamentous and surrounded by a mucilaginous sheath; active motility is not observed, but weak oscillation of trichome tips is noted. Vegetative cells are blue-green, 2.6–5.0 µm wide; cell length is 2–3 times greater than cell width. Heterocysts are absent. Akinetes are absent. Reproduction occurs by binary cell division in one plane. In liquid medium, the strain forms a blue-green slightly mucilaginous suspension with an amorphous sediment that is easily resuspended by shaking. On agarized Gromov medium, it forms blue-green spherical clusters of trichomes. The culture develops independently of season.

Cultivation

• Nutrient media: Gromov's, Allen's, BG-11 (liquid and agarized)
• Temperature: 25–28 °C
• Light intensity: 30–40 µmol photons/m²/s
• Photoperiod: continuous illumination
• pH: 7.5–8.0

Physiological Characteristics

Mesophilic homocytous filamentous cyanobacterium. It does not form heterocysts or akinetes. Under the experimental conditions, nitrogen-fixing activity was not detected, which corresponds to the absence of heterocysts in this morphotype.

Long-term Preservation Conditions

stored on agarized BG-11 medium at 12–14 °C under low illumination of 4–6 µmol photons/m²/s. Maximum storage period without subculturing: 2–3 months.

Genetic Information

A fragment of the 16S rRNA gene was amplified. Based on morphological and phylogenetic analysis, the strain was identified as Oscillatoria brevis SH-12. GenBank accession number: MZ090011.

Field of Application

The strain is used in experimental studies. It is promising for biotreatment, bioremediation, and algal-cyanobacterial biofilm formation. It may also be used as a phototrophic model culture among non-heterocystous filamentous cyanobacteria.

Bibliography

1. Sadvakasova A.K. et al. Influence of Mo and Fe on Photosynthetic and Nitrogenase Activities of Nitrogen-Fixing Cyanobacteria under Nitrogen Starvation. Cells. 2022; 11(5):904. DOI: 10.3390/cells11050904.

Patents and Other Intellectual Property

none.