Chlorella vulgaris SP 4

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: sewage treatment facilities of Almaty city
• Habitat: wastewater
• Isolator(s): Zayadan B.K., Akmukhanova N.R. in the biotechnology laboratory of Al-Farabi KazNU
• Year of accession to the collection: 2001
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
• Genetic features: Wild type
• Strain condition: axenic

Morphology and Structure

Cells are spherical and covered with a very thin wall. Cell diameter ranges from 1.7 to 5.2 µm. Inside the cell, there is a light-colored, girdle-shaped chromatophore. The nucleus is not visible in living cells.

Cultivation

• Culture medium: Tamiya mineral medium (liquid and agarized)
• Temperature: 24–28 °C
• Light intensity: 50–100 µmol photons/m²/s
• Light regime: 16:8 h (light/dark)
• pH of the medium: 6.5

Physiological Characteristics

Mesophile, autotroph. Under optimal conditions for biomass production (with continuous CO₂ supply) over 7–8 days, the composition of the dry biomass (at a concentration of 2.0–3.0 g/l) is as follows: proteins — 45–50%, carbohydrates — 40–45%.

Long-term Preservation Conditions

Stored on liquid and 1.5–2.0 % agarized Tamiya mineral medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

Deposited in 2003 in the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration number - A-RKM 0118.

Field of Application

In fundamental research and biotechnology.

Bibliography

1. Заядан, Б. К. (2001). Жасыл балдырлар Chlorella клеткаларының ауыр металдарға төзімділігі мен олардың биомассасындағы цистеин мөлшері арасындағы қатынасты зерттеу нәтижелері. Вестник КазГУ. Серия биологическая, (2), 117–121.

Patents and Other Intellectual Property

Patent for invention No. 25373. Application No. 2010/1358.1 dated 05.11.2010. Publication date: 20.12.2011, bulletin No. 12. Title: "Strain of unicellular green algae Chlorella vulgaris SP4 for biomass production." Patent authors: Musaldinov Tulegen Botpaevich; Zayadan Bolatkhan Kazykhanovich; Zhambakin Kabyl Zhaparovich; Akmukhanova Nurziya Rakhmedievna; Sadanov Amankeldi Kurbanovich.